年度 95
計劃編號 NSC95-2221-E-005-056-
研究學門 環境工程
中文計劃名稱 紫色不含硫光合作用細菌之聚磷酸鹽累積相關酵素與基因表現之探討
執行期限 2006-08-01～2007-07-31
主持人 洪俊雄
職稱
預算 1214000
中文摘要 紫色不含硫光合作用細菌已成功被應用於分解高濃度COD之豬糞尿廢水及養殖廢水上，更發現這類細菌能自廢水中攝取磷酸鹽，進而以聚磷酸鹽形式累積於細胞體內，累積量甚至可達一般除磷菌的二倍至三倍以上，其累積磷酸鹽之模式較為特殊，至目前為止顯少被文獻所提及。本研究計畫探討紫色不含硫光合作用細菌Rhodopseudomonas palustris GN11於厭氧光照培養下基因表現與除磷能力之關係。Rhodopseudomonas palustris GN11於此條件下，可攝取培養基中磷酸鹽進而轉變成聚磷酸鹽形式累積於胞內，於70小時內培養基中磷酸鹽濃度由220 mg/L下降為188 mg/L，經培養時間的增加，胞內聚磷酸鹽由低分子量聚磷酸鹽轉變成高分子量聚磷酸鹽。而菌株在不同生長時期中磷酸鹽消耗、聚磷酸鹽形成與PHB消長，皆藉由基因表現量的分析獲得證實。菌株在對數生長期時能量的來源主要來自光合作用；當進入生長穩定期前期，則分解PHB作為部分能量來源，當進入生長穩定期後期，累積於胞內之聚磷酸鹽被分解獲得能量，此時光合作用對紫色不含硫光合作用細菌已不如培養初期時重要。透過蛋白質分析結果可發現，仍有許多酵素值得在建立紫色不含硫光合作用細菌特有除磷模式時進行探討。
英文摘要 Polyphosphate-accumulating ability of the phototrophic purple nonsulfur bacteria was found to be twice stronger than other PAOs in illuminative anaerobic condition, and this model of biological phosphorus removal were different with the PAOs model described by scientists. It was also demonstrated that bioaugmentation of purple nonsulfur bacteria with high polyphosphate accumulating ability into lab-scale activated sludge system could enhance the overall efficiency of phosphorus removal. All of these results prompted an interesting research subject on the phosphorus accumulating mechanism the purple nonsulfur bacteria. In this research, polyphosphate accumulating phenomenon performed by the purple nonsulfur bacteria and the pathway of this polyphosphate metabolism were studied. Results of this study showed that Rhodopseudomonas palustris GN11 could accumulate polyphosphate by assimilating phosphorus from 220 mg/L to 188 mg/L under illuminative anaerobic condition. Low-molecular-weight polyphosphate was found to be the predominant polyphosphate form at bacterial exponential phase and it converts to high-molecular-weight polyphosphate with incubation time. From the relative quantification of specific gene expression, it was demonstrated that energy from photosynthesis is important when bacteria was in exponential phase. Expression of PHB synthase gene and PHB depolymerase gene also showed that PHB degradation could provide partial energy for growth while entering initial stationary phase. Polyphosphate was suggested to be the energy and phosphorus sources at later stationary phase from the results of ppk and ppx gene expressions. Photosynthesis seems to be absent and less important in this phase from pufM gene expression. Furthermore, results of 2D protein electrophoresis suggests that there are more expressed proteins should be study while Rhodopseudomonas palustris GN11 accumulating polyphosphate. Studies on these protein could helps to establish a reliable phosphorus removal model belongs to purple nonsulfur bacteria Rhodopseudomonas palustris GN11.
中文關鍵字 生物除磷
英文關鍵字 purple nonsulfur bacteria, phosphorus removal, gene expression Β????? ?? ????????????????????????????????? ??????????????????????????????????? ????Κ????????????Κ?Β??Ο???????????? ???????????????????? SBR ???????????? ????????[1]????????????????????????? ?????????????????Ψ??????????η????η? ?[2]???????Ψ???????????????????????? ε?????[1]??????????????????????????? ??????????????????????????????????? ???????????? ???? ????????????????????Π???????????? ?????????????????????-Rhodopseudomonas palustris GN11 ???????????????????????????????? ???????????Π??????????Π???????????? ???????????????? GN11 ???????????????? ?????????????????????????????? ???? ????????????????????????????????? 3 ???????????????????????[4]?????????? ?????????????????????????Π???????? ?Κ??????????? 15~26?[5]???????????? 5-8??? ?????[6, 7]??????????????????????????? ??????????????????????????????????? ??????????? ????????????????????Π???????????? ????????????????????????
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